Krystal Morton

Abstract: Early Response Genes (ERG) are activated rapidly in response to stimuli.
Stimulated neurons incur DNA double strand breaks (DSBs) within the promoters of early response genes (ERGs), such as Fos. Stimulation-dependent break formation is mediated by Topoisomerase 2ß (Top2ß) and is required for ERG induction. The focus of the study was to determine if low repair fidelity of the DSB in the promoter region would inhibit future ERG induction. Etoposide was used to force Top2ß mediated DSBs in human embryonic kidney cells (HEK293T). These breaks were then repaired and then a second round of etoposide was given. Then CRISPR Cas-9 was used to create a break 1kb away from the transcription start site in HEK293T cells. qPCR results did not show an inhibition of Fos induction following a CRISPR medicated DSB. The results of the study indicated that Top2ß mediated break formation can occur in the same location repeatedly, even in response to stimulation induced DSBs, and potentiates subsequent induction and progressive potentiation of ERG. It also supports the idea Fos gene induction caused by DSBs does not require the break to be generated by Top2ß. Lastly, the use of CRISPR pointed to the idea that mutation accrual in promoter region of Fos leads to potentiated gene transcription following stimulation dependent DSBs.

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​What does research mean to you? 
To me research means having the ability to create new knowledge. It allows you to learn newly discovered information and expand upon it. This is a type of thinking that is not always taught within a classroom. Research gives you the freedom the combine creativity with knowledge.

Tell us about your journey.
I entered university interested in exploring research. I gained experience in social science research early on but was unable to join a neuroscience lab until the Green Fellowship offered me the opportunity. I found that I really enjoyed the lab environment and my lab members helped me learn what it means to think critically about a research project. The experience I gained through the Green Fellowship encourages me to pursue research opportunities in the future even if I don't want it to be my career.
To me research means having the ability to create new knowledge. It allows you to learn newly discovered information and expand upon it. This is a type of thinking that is not always taught within a classroom. Research gives you the freedom the combine creativity with knowledge.

What was your favorite part of the program?
My favorite part was getting to work with my PI. I always looked forward to our weekly meetings because I left with a greater understanding and appreciation of not only my project, but of science in general. He emphasized the importance of academic autonomy and taught me how to analyze research in a way that leads you to think of future directions. 

What was the biggest thing that you learned?
How to ask for help. As someone without bench experience I entered an environment where I felt like I knew nothing. I had to learn quickly to set aside my ego and ask questions because that is the only way to learn.

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Advice for Future Green Fellows

Enter the program with an open mind and expect to feel lost for the first couple weeks.